Whole-Exome Sequencing Reveals Somatic Mutations in HRAS and KRAS , which Cause Nevus Sebaceus

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Whole exome sequencing reveals somatic mutations in HRAS and KRAS which cause nevus sebaceus

Jonathan L. Levinsohn1, Li C. Tian1, Lynn M. Boyden2, Jennifer M. McNiff1, Deepak Narayan3, Erin S. Loring2, Duri Yun4, Jeffrey L. Sugarman5, John D. Overton2,6, Shrikant M. Mane2,6, Richard P. Lifton2,6,7, Amy S. Paller4, Annette M. Wagner4, Richard J. Antaya1,8, and Keith A. Choate1 1Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut 2Department of Genetics,...

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Background: Nephronophthisis (NPHP) is a progressive tubulointestinal kidney condition that demonstrates an AR inheritance pattern. Up to now, more than 20 various genes have been detected for NPHP, with NPHP1 as the first one detected. X-prolyl aminopeptidase 3 (XPNPEP3) mutation is related to NPHP-like 1 nephropathy and late onset NPHP. Methods: The proband (index patient) had polyuria, polyd...

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Urachal adenocarcinoma is a rare bladder malignancy arising from the urachal remnant. Given its rarity and the lack of knowledge about its genetic characteristics, optimal management of this cancer is not well defined. Practice patterns vary and outcomes remain poor. In order to identify the genomic underpinnings of this malignancy, we performed whole exome sequencing using seven tumor/normal p...

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Mosaic activating RAS mutations in nevus sebaceus and nevus sebaceus syndrome

Exome sequencing Genomic DNA was isolated using the Qiagen Blood/Tissue kit (Qiagen, Valencia, CA). Exome capture was performed with the Agilent SureSelect All-Exon v2 kit, and 100 bp paired-end library reads were generated on Illumina HiSeq 2000 to a 50-fold target average depth of coverage. Alignment of reads to the human genome was performed with DNAnexus (www.dnanexus.com) and bwa (Li and D...

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Identification of Somatic KRAS Mutation in a Korean Baby with Nevus Sebaceus Syndrome

Nevus sebaceus is a benign hamartoma of the epidermis, hair follicles, and sebaceous and apocrine glands. It typically appears as a yellow-orange to pink, finely papillomatous alopecic plaque that is often oval or linear. It usually presents at birth, preferentially affects the scalp and face, and is not uncommon, occurring in approximately 1 in 1,000 live births [1]. A recent study by Groesser...

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ژورنال

عنوان ژورنال: Journal of Investigative Dermatology

سال: 2013

ISSN: 0022-202X

DOI: 10.1038/jid.2012.379